Publish Time: 2024-08-21 Origin: Site
Botulax, a popular botulinum toxin type A brand, is widely used for cosmetic and therapeutic purposes, including wrinkle reduction, facial contouring, and treatment of hyperhidrosis. To ensure safe and effective treatment, proper reconstitution and dilution of Botulax is essential. This guide provides a detailed overview of how to reconstitute Botulax 100, 200, and 300 units, along with dilution charts and best practices for clinical use.
Botulax is a purified botulinum toxin type A product developed in South Korea. It is known for its high safety profile, stable formulation, and affordability, making it a popular choice among aesthetic professionals worldwide. It is available in 100 units (U), 200 units (U), and 300 units (U) vials.
The amount of saline used determines the final concentration (units per 0.1 mL). This affects how many injection points you can cover and how precise the dosing will be.
Common Dilution Ratios:
You can adjust the dilution depending on the treatment area (e.g., forehead, crow's feet, jawline, underarms) and desired intensity.
Before beginning the reconstitution process, ensure that you have all the necessary materials and equipment:
● Botulax vial: The product typically comes in a vial containing 100 units of botulinum toxin type A.
● This is the recommended diluent for reconstituting the Botulax powder.
● Syringe (1-3 mL): A sterile syringe is needed for drawing and injecting the saline into the vial.
● Alcohol swabs: For sterilizing the rubber stopper of the Botulax vial and cleaning your hands or gloves before starting.
● Insulin needles (optional): For more precise administration when injecting smaller areas.
● Ensure that your working environment is clean, with sanitized surfaces, and that you are wearing gloves to maintain sterility during the process.
Start by washing your hands thoroughly with soap and water or applying hand sanitizer. Wearing gloves is highly recommended for maintaining sterility.
Arrange your materials and equipment on a clean surface, ensuring everything is within easy reach.
Draw the desired amount of sterile saline (0.9% sodium chloride) into the syringe. For a standard dilution, the most commonly used ratio is 2.5 mL or 2.0 mL of saline for a 100-unit Botulax vial. However, different dilution ratios are used depending on the intended application. Please refer to the botulax dilution chart above to determine the amount of saline required
Gently wipe the rubber stopper of the Botulax vial with an alcohol swab to disinfect it.
Insert the needle of the syringe containing the saline into the center of the stopper. It is recommended to inject the saline slowly and at an angle to minimize foaming, which can occur if the saline is injected too quickly or directly onto the powder.
While injecting, allow the saline to run down the inner walls of the vial, further reducing the risk of foaming. The goal is to achieve a clear and consistent solution without bubbles.
After injecting the saline, gently swirl the vial in your hand to mix the solution. Avoid vigorous shaking, as this can denature the botulinum toxin and reduce its effectiveness.
Let the vial sit at room temperature for a few minutes to allow any bubbles to settle before drawing the solution into your injection syringe.
After the mixture has settled, use an insulin syringe or another fine-gauge needle to draw the required dosage from the vial.
If administering multiple injections, ensure that you regularly check the vial for clarity and consistency of the solution. Proper handling and gentle mixing before each draw are essential for even distribution.
Maintain Sterility: Always use sterile gloves, equipment, and materials. Contamination can lead to infections or compromised results.
Proper Storage: Once reconstituted, Botulax should be stored in a refrigerator (2-8°C). It is generally recommended to use the solution within 24 hours, although some practitioners may extend this to 72 hours, depending on clinical guidelines and patient needs.
Consistent Technique: Following the same mixing protocol each time ensures consistent results. Variations in technique can lead to different concentrations, affecting the efficacy and duration of treatment.
Patient Safety: Always check the patient's medical history and any contraindications. Botulinum toxin should only be administered by licensed and trained professionals who understand both the clinical and aesthetic implications.
Shaking the Vial: Shaking vigorously during reconstitution can denature the toxin. Always gently swirl instead.
Injecting Saline Too Quickly: Rapid injection of saline can create foam, leading to inconsistent concentrations and ineffective results.
Incorrect Dilution Ratios: Using the wrong saline volume can lead to either overly concentrated or overly diluted solutions, affecting the overall treatment outcome.
Not Waiting for the Solution to Settle: Drawing the solution too soon after mixing can introduce air bubbles into the injection, leading to uneven distribution in treated areas.
Customizing Dilution Ratios: Depending on the specific treatment area (e.g., crow's feet, forehead lines, neck bands), different dilution ratios might be more effective. For example, a higher concentration may be better for smaller, targeted areas, while a lower concentration is often used for larger regions.
Monitoring Patient Reactions: During the initial session, closely monitor the patient's reaction to the treatment. Adjustments in dosage or dilution might be necessary for subsequent sessions based on the patient's response.
Documenting Procedures: Keep detailed records of the dilution ratios, injection sites, and patient outcomes. This information is invaluable for fine-tuning future treatments and ensuring consistency.
Properly reconstituting and diluting Botulax is essential for achieving precise results, minimizing side effects, and maximizing product efficiency. Whether you're using Botulax 100U, 200U, or 300U, always follow sterile practices, adjust dilutions according to the treatment area, and use professional judgment for each patient's needs.
If you're looking to buy Botulax at wholesale prices or need more product guidance, feel free to contact us.
Can I use the same dilution for all areas?
No. Use higher concentrations (e.g., 10 U/0.1 mL) for strong muscles like masseters, and lower concentrations (e.g., 2–3 U/0.1 mL) for fine lines or superficial areas.
Botulax Dilution Chart
Vial Size | Saline Volume | Units per 0.1 mL | Best Use Areas |
100U | 1 mL | 10 U | Strong muscles, masseter |
100U | 2.5 mL | 4 U | Glabella, forehead |
100U | 5 mL | 2 U | Crow's feet, fine lines |
200U | 4 mL | 5 U | Hyperhidrosis, masseter |
300U | 6 mL | 5 U | Larger areas, multiple sites |
Is Botulax safe after reconstitution?
Yes, if stored correctly at 2°C–8°C and used within 24 hours. Avoid prolonged storage to maintain potency.