You are here: Home New » Blog » Blog » Mechanism of Action » How Long Does Botulax Last Once Mixed

How Long Does Botulax Last Once Mixed

facebook sharing button
twitter sharing button
line sharing button
wechat sharing button
linkedin sharing button
pinterest sharing button
whatsapp sharing button
kakao sharing button
snapchat sharing button
telegram sharing button
sharethis sharing button

Botulax is supplied as a lyophilized powder, which must be reconstituted with a sterile saline solution before administration. The reconstitution process involves adding a specified amount of saline to the vial containing Botulax powder, which should be done under aseptic conditions to prevent contamination. The concentration of the solution can vary depending on the intended use and the practitioner’s preference.

How Long Does Botulax Last Once Mixed

Reconstitution of Botulax

Reconstitution refers to the process of mixing the botulinum toxin powder with a diluent, typically saline, to prepare it for injection. This process is delicate and must be done under sterile conditions to maintain the product's integrity and efficacy. The recommended dilution varies depending on the intended use, with common concentrations ranging from 1.0 to 2.5 mL of saline per vial of Botulax.

Stability After Reconstitution

Once reconstituted, the stability of Botulax is influenced by several factors, including storage temperature, exposure to light, and time elapsed since reconstitution. According to the manufacturer’s guidelines and various studies, Botulax remains effective for a certain period post-reconstitution, provided it is stored correctly.

Factors Influencing the Stability of Mixed Botulax

Several factors influence the stability and longevity of Botulax once it has been reconstituted:

Temperature: The temperature at which mixed Botulax is stored plays a crucial role in its stability. Botulax should be stored in a refrigerator at a temperature of 2-8°C (36-46°F). Exposure to higher temperatures can degrade the toxin, reducing its efficacy.

Light Exposure: Botulinum toxin is sensitive to light. Therefore, mixed Botulax should be stored in its original vial, which is typically opaque, and kept away from direct light to maintain its potency.

Time: The time elapsed since reconstitution significantly affects Botulax’s effectiveness. Although the manufacturer may provide specific guidelines, a general consensus among medical professionals is that reconstituted Botulax should ideally be used within 24 hours. Some studies suggest that it can remain effective for up to 2-4 weeks if stored properly, but its potency diminishes over time.

Contamination Risk: Ensuring that the reconstitution process is conducted under sterile conditions is paramount. Any contamination can not only reduce the efficacy of the toxin but also pose serious health risks.

Best Practices for Using Reconstituted Botulax

To maximize the effectiveness and safety of Botulax after mixing, the following best practices should be adhered to:

Aseptic Technique: Always use sterile equipment and maintain aseptic conditions during reconstitution to prevent contamination.

Proper Storage: Immediately store the reconstituted Botulax in a refrigerator at 2-8°C. Do not freeze the solution, as this can damage the toxin’s structure.

Timely Use: Aim to use the reconstituted Botulax within 24 hours to ensure maximum potency. If extended use is necessary, be aware that efficacy may decrease over time.

Minimize Light Exposure: Keep the vial in its original packaging or another light-protective container to shield it from light.

Clinical Implications

Understanding the stability and duration of reconstituted Botulax has significant clinical implications. The efficacy of Botulax treatments, whether for aesthetic purposes such as wrinkle reduction or medical conditions like muscle spasticity, depends on the potency of the product at the time of injection.


Using Botulax within the recommended timeframe ensures that patients receive the maximum benefit from the treatment. The toxin's ability to block neuromuscular transmission is most effective when it is fresh, leading to better clinical outcomes.


Adhering to storage guidelines not only maintains efficacy but also ensures safety. Degraded or contaminated Botulax can lead to adverse effects or infection. By following best practices, practitioners can minimize these risks and provide safe, effective treatments.


How Long Does Botulax Last Once Mixed

The duration of Botulax after reconstitution is influenced by several factors, including temperature, light exposure, and time. For optimal results, it is advisable to use reconstituted Botulax immediately or within a short timeframe, typically 24 hours to 7 days. Proper storage in a refrigerated environment and protection from light are essential to maintain the product's potency and ensure patient safety. By understanding and adhering to these guidelines, practitioners can deliver effective and safe Botulax treatments, enhancing patient satisfaction and clinical outcomes.






WhatsApp: +8617730567046
Tel: +8617730567046
Add: Rm 1407, Block B, Wanda Bldg, No. 245 Jianhua South Street, Yuhua Dist, Shijiazhuang, Hebei, China
Copyright © 2024 Dermax Medical Technology (Hebei) Co., Ltd. All rights reserved.